6ORC

Crystal structure of Sel1 repeat protein from Oxalobacter formigenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-28
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	73.301, 75.479, 78.344
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.662 - 2.980
R-factor	0.2101
Rwork	0.208
R-free	0.24760
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.020
High resolution limit [Å]	2.970	8.050	2.970
Rmerge	0.112	0.101	0.959
Rmeas	0.125	0.114	1.085
Rpim	0.053	0.051	0.496
Total number of observations	51377
Number of reflections	9035	503	422
<I/σ(I)>	11.7
Completeness [%]	99.4	96.2	98.6
Redundancy	5.7	5.1	4.4
CC(1/2)		0.939	0.641

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	0.1M Tris-HCl, 20 % PEG1000