6OR1
Human LRH-1 bound to the agonist 2N and a fragment of the Tif2 coregulator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2018-03-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 46.658, 46.658, 222.696 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.033 - 2.174 |
R-factor | 0.2017 |
Rwork | 0.200 |
R-free | 0.23870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB 5L11 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.422 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
High resolution limit [Å] | 2.174 | 4.740 | 2.174 |
Rmerge | 0.163 | 0.117 | |
Rmeas | 0.167 | 0.119 | |
Rpim | 0.036 | 0.026 | 0.309 |
Total number of observations | 280833 | ||
Number of reflections | 13301 | 1556 | 1170 |
<I/σ(I)> | 3.4 | ||
Completeness [%] | 96.6 | 99.6 | 87.9 |
Redundancy | 21.1 | 20.4 | 13 |
CC(1/2) | 0.996 | 0.697 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.05M sodium acetate (pH 4.6), 5-11% PEG4000, 0-10% glycerol. Microseeding with LRH-1-RJW100 crystals. |