6OPM
Casposase bound to integration product
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792966, 0.9791605,0.9680223 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 106.390, 106.390, 423.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.890 - 3.100 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.25600 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 45362 | 3309 |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 99.8 | |
| Redundancy | 14.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.15-0.185 M calcium acetate, 84 mM sodium acetate, pH 3.8-4.4, 7.5-9.0% PEG8000, 0.5 M potassium chloride |
