6OPJ
Menin in complex with peptide inhibitor 25
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-12-07 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.867, 78.145, 123.702 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.976 - 1.501 |
R-factor | 0.169817677969 |
Rwork | 0.168 |
R-free | 0.19743 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4x5y |
RMSD bond length | 0.005 |
RMSD bond angle | 0.910 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | phenix.refine (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.560 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.083 | 0.574 |
Number of reflections | 75018 | 7941 |
<I/σ(I)> | 18.15 | |
Completeness [%] | 98.9 | |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M lithium sulfate, 0.1 M HEPES, pH 7.5, 25% w/v PEG3350 |