6OPJ
Menin in complex with peptide inhibitor 25
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-12-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.867, 78.145, 123.702 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.976 - 1.501 |
| R-factor | 0.169817677969 |
| Rwork | 0.168 |
| R-free | 0.19743 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4x5y |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.910 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | phenix.refine (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.560 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.083 | 0.574 |
| Number of reflections | 75018 | 7941 |
| <I/σ(I)> | 18.15 | |
| Completeness [%] | 98.9 | |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M lithium sulfate, 0.1 M HEPES, pH 7.5, 25% w/v PEG3350 |
