Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 116.385, 116.385, 58.194 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.149 - 2.501 |
| R-factor | 0.1733 |
| Rwork | 0.170 |
| R-free | 0.23020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.929 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.150 | 2.591 |
| High resolution limit [Å] | 2.501 | 2.501 |
| Rmerge | 0.141 | 0.596 |
| Rmeas | 0.147 | 0.654 |
| Rpim | 0.039 | 0.259 |
| Number of reflections | 15679 | 1305 |
| <I/σ(I)> | 15.5 | 2.7 |
| Completeness [%] | 98.1 | 83.1 |
| Redundancy | 13.4 | 5.6 |
| CC(1/2) | 0.996 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 10% PEG 20000, 0.1M MES, pH 6.7 |
