6OP8

S. pombe Ubc7/U7BR complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
Unit cell lengths	26.715, 79.047, 94.521
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.260 - 1.703
R-factor	0.1567
Rwork	0.155
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jqu
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.279	1.764
High resolution limit [Å]	1.703	1.703
Rmerge	0.056	0.223
Rmeas	0.062	0.246
Rpim	0.025	0.102
Number of reflections	22673	2195
<I/σ(I)>	25.94	9.05
Completeness [%]	99.4	98.12
Redundancy	5.7	5.7
CC(1/2)	0.999	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	100 mM TRIS, pH 8.0, 200 mM MgCl2, 30% PEG4000