6OO0
Crystal structure of bovine Fab NC-Cow1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.060, 69.560, 95.610 |
| Unit cell angles | 90.00, 106.99, 90.00 |
Refinement procedure
| Resolution | 28.865 - 2.100 |
| R-factor | 0.2037 |
| Rwork | 0.202 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ilt |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.583 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.900 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.162 | 1.460 |
| Rpim | 0.078 | 0.693 |
| Number of reflections | 30513 | 2243 |
| <I/σ(I)> | 6 | 1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.1 | 4.1 |
| CC(1/2) | 0.991 | 0.303 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1M sodium cacodylate, 40% methylpentanediol, 5% PEG 8000 |
