6ONW
Crystal structure of Sel1 repeat protein from Oxalobacter formigenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-06 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.773, 74.079, 194.589 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.990 - 2.951 |
R-factor | 0.2133 |
Rwork | 0.211 |
R-free | 0.25930 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.425 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
High resolution limit [Å] | 2.950 | 8.000 | 2.950 |
Rmerge | 0.169 | 0.063 | 0.730 |
Rmeas | 0.189 | 0.071 | 0.833 |
Rpim | 0.081 | 0.031 | 0.393 |
Number of reflections | 21711 | 1215 | 992 |
<I/σ(I)> | 4.3 | ||
Completeness [%] | 98.7 | 98.6 | 92.8 |
Redundancy | 5.2 | 4.9 | 3.9 |
CC(1/2) | 0.991 | 0.597 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.1M Tris-Cl, 30% PEG1000 |