6ONT

Structure of the Francisella response regulator 1452 receiver domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	I 41 3 2
Unit cell lengths	128.726, 128.726, 128.726
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.512 - 1.803
R-factor	0.1844
Rwork	0.182
R-free	0.20190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uic
RMSD bond length	0.005
RMSD bond angle	0.782
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	17102
<I/σ(I)>	83.425	2.205
Completeness [%]	100.0	99.94
Redundancy	24.7	8
CC(1/2)	0.083	0.502

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.2 M Calcium Chloride, 0.1 M HEPES pH 7.5, 28% PEG 400