6ONQ
Crystal structure of c-type cytochrome XoxG from Methylobacterium extorquens AM1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-08-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.6984 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 95.841, 95.841, 80.411 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.500 - 2.710 |
R-factor | 0.1963 |
Rwork | 0.194 |
R-free | 0.24840 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.970 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.760 |
High resolution limit [Å] | 2.710 | 2.710 |
Rmerge | 0.124 | 1.242 |
Rpim | 0.020 | 0.256 |
Number of reflections | 6337 | 305 |
<I/σ(I)> | 39 | |
Completeness [%] | 98.6 | |
Redundancy | 37.7 | |
CC(1/2) | 0.999 | 0.898 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | .2 M sodium acetate, 0.1 M phosphate-citra te pH 4.2, and 20% (w/v) PEG 8000 |