6ONB

Crystal Structure of the ZIG-8-RIG-5 IG1-IG1 heterodimer, monoclinic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	120
Detector technology	PIXEL
Collection date	2019-02-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	C 1 2 1
Unit cell lengths	87.460, 79.103, 89.977
Unit cell angles	90.00, 94.22, 90.00

Refinement procedure

Resolution	89.733 - 1.696
R-factor	0.1755
Rwork	0.174
R-free	0.20730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6on9
RMSD bond length	0.006
RMSD bond angle	0.821
Data reduction software	XDS (20180808)
Data scaling software	XDS (20180808)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.15rc3_3435)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.800
High resolution limit [Å]	1.696	1.700
Rmerge	0.047	0.587
Rmeas	0.055	0.692
Number of reflections	65564	10243
<I/σ(I)>	14.15	1.62
Completeness [%]	96.8	93.8
Redundancy	3.6	3.4
CC(1/2)	0.999	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	0.2 M lithium sulfate, 0.1 M sodium cacodylate, pH 6.5, 30% PEG 400