6ONA

Crystal structure of Influenza hemagglutinin from strain A/Hickox/JY2/1940

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-21
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	C 1 2 1
Unit cell lengths	108.560, 102.250, 160.950
Unit cell angles	90.00, 91.07, 90.00

Refinement procedure

Resolution	45.388 - 1.950
R-factor	0.1936
Rwork	0.193
R-free	0.22970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mya
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3409)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.388	45.388	2.000
High resolution limit [Å]	1.950	8.720	1.950
Rmerge	0.077	0.026	0.875
Rmeas	0.085	0.029	0.974
Total number of observations	661978
Number of reflections	127783	1497	9450
<I/σ(I)>	12.85	38.59	2.13
Completeness [%]	99.9	98.9	99.9
Redundancy	5.18	4.884	5.301
CC(1/2)	0.999	0.998	0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	287	0.1 uL 9.5 mg/mL SEC-purified InvbQ.18715.a.KN11.PD38349 in 2 5 mM Tris, pH 8.5, 150 mM sodium chloride + 0.1 uL mother liquor (0.1 M sodium citrate, pH 5.5, 0.1 M magnesium chloride, 34% PEG400), flash-frozen, crystal ID 308018c7 data set les6-6