6OML

Human BMP chimera BV261

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-16
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	67.779, 67.779, 148.011
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.980 - 2.700
R-factor	0.2017
Rwork	0.199
R-free	0.26540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.290
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	BUSTER (2.11.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.980	49.980
High resolution limit [Å]	2.700	2.700
Number of reflections	10061	10061
<I/σ(I)>	2
Completeness [%]	100.0
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	50mM Ammonium Sulfate, 100mM Bis-Tris pH 6.5, 30% Pentaerythritol Ethoxylate