6OL6

Structure of iglb12 scFv in complex with anti-idiotype ib2 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	135.688, 95.895, 66.741
Unit cell angles	90.00, 96.15, 90.00

Refinement procedure

Resolution	49.105 - 2.600
R-factor	0.174
Rwork	0.171
R-free	0.23060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hzh
RMSD bond length	0.002
RMSD bond angle	0.559
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Number of reflections	26000	1171
<I/σ(I)>	9.2
Completeness [%]	99.5	91.1
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	13.4% PEG 8000, 13.4 PEG 400, 0.1 M MgCl2, 0.1 M Tris pH 8.5