6OL5

Structure of iglb12 Fab in complex with anti-idiotype ib3 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 43 2 2
Unit cell lengths	75.551, 75.551, 389.714
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.408 - 2.900
R-factor	0.2021
Rwork	0.200
R-free	0.24990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hzh
RMSD bond length	0.003
RMSD bond angle	0.624
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Number of reflections	26152	1174
<I/σ(I)>	10.3
Completeness [%]	99.5	93.4
Redundancy	17.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2 M Ammonium phosphate monobasic, 0.1 M Tris pH 8.5, and 50%(+/-) 2-methyl-2,4-pentanediol