6OKN

OX40R (TNFRSF4) bound to Fab 1A7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-22
Detector	RAYONIX MX300HE
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	121.590, 121.590, 198.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.490 - 3.250
R-factor	0.194
Rwork	0.192
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.110
Data reduction software	MOSFLM
Data scaling software	Aimless (0.5.28)
Phasing software	PHASER
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.490	29.490	3.470
High resolution limit [Å]	3.250	9.190	3.250
Rmerge	0.166	0.078	0.692
Rmeas	0.172	0.082	0.733
Rpim	0.047	0.023	0.234
Number of reflections	24096	1174	4263
<I/σ(I)>	9.2
Completeness [%]	99.8	97.2	99.4
Redundancy	13.3	12.5	9.1
CC(1/2)	0.997	0.997	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	tbd