Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 193 |
Detector technology | CCD |
Collection date | 2012-02-29 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.953695 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.270, 85.310, 85.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.152 - 1.730 |
R-factor | 0.2112 |
Rwork | 0.209 |
R-free | 0.24750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mis |
Data reduction software | xia2 |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.610 | 1.820 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.745 | |
Rmeas | 0.293 | 0.802 |
Rpim | 0.111 | 0.295 |
Number of reflections | 51255 | 7386 |
<I/σ(I)> | 5.1 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.2 | 7.3 |
CC(1/2) | 0.950 | 0.776 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | A reservoir solution comprised of 72% (w/v) MPD, 0.1 M Tris, pH 8.8. Using an equal reservoir to protein ratio, crystals were observed after 1 day and grew to maximal size after 2 weeks |