6OJY

Methylated PilT4 from Geobacter metallireducens bound to sulfate: C3ocococ conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	115.800, 119.010, 178.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.558 - 3.300
R-factor	0.2107
Rwork	0.209
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jvv
RMSD bond length	0.005
RMSD bond angle	0.894
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.558	3.400
High resolution limit [Å]	3.300	3.300
Rpim	0.067	0.400
Number of reflections	37753	3658
<I/σ(I)>	12.6
Completeness [%]	100.0
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25 mM Hepes pH 7 75 mM NaCl 5 % (v/v) glycerol 100 mM ammonium sulphate 50 mM Bis-Tris-HCl pH 6.5 12.5% (w/v) PEG3350