6OJL
Structure of YePL2A R194K in Complex with Pentagalacturonic Acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-03 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.510, 96.080, 127.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.507 - 1.500 |
| R-factor | 0.1686 |
| Rwork | 0.168 |
| R-free | 0.18620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v8k |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.791 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.530 | 1.517 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.045 | 0.622 |
| Number of reflections | 114656 | 3554 |
| <I/σ(I)> | 17.82 | 2.63 |
| Completeness [%] | 99.6 | 99.87 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | PEG 1500, Sodium Acetate |
