6OJJ
Structure of ScAtg3 with truncations in N-terminal and flexible region (FR)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 92.078, 44.557, 66.200 |
Unit cell angles | 90.00, 102.98, 90.00 |
Refinement procedure
Resolution | 26.408 - 2.406 |
R-factor | 0.2146 |
Rwork | 0.213 |
R-free | 0.24320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dyt |
RMSD bond length | 0.002 |
RMSD bond angle | 0.674 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rpim | 0.094 | 0.527 |
Number of reflections | 10013 | 482 |
<I/σ(I)> | 12.4 | 1.3 |
Completeness [%] | 97.9 | 92.7 |
Redundancy | 3.4 | 2.8 |
CC(1/2) | 0.587 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277.15 | 9% PEG3350, 100 mM HEPES pH 7.2, 150 mM L-Malic acid. |