6OJ1

Crystal Structure of Aspergillus fumigatus Ega3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-19
Detector	RAYONIX MX300HE
Wavelength(s)	0.97947
Spacegroup name	P 21 21 21
Unit cell lengths	44.640, 47.310, 152.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.186 - 1.760
R-factor	0.1634
Rwork	0.162
R-free	0.19190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2aam
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	Arcimboldo (Shredder)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.186	1.820
High resolution limit [Å]	1.760	1.760
Rmerge	0.063	0.595
Rmeas	0.066	0.620
Rpim	0.017	0.180
Number of reflections	32553	2863
<I/σ(I)>	28.1	4.27
Completeness [%]	99.8	88.7
Redundancy	14	11.4
CC(1/2)	1.000	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M potassium iodide, 20 % (v/v) PEG 3350