6OIL

Crystal structure of human VISTA extracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	29.169, 64.089, 37.695
Unit cell angles	90.00, 91.73, 90.00

Refinement procedure

Resolution	29.156 - 1.850
R-factor	0.1821
Rwork	0.180
R-free	0.22260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Rosetta generated models
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.156	29.156	1.900
High resolution limit [Å]	1.850	8.270	1.850
Rmerge	0.096	0.039	1.467
Rmeas	0.101	0.042	1.562
Total number of observations	107740
Number of reflections	11704	144	843
<I/σ(I)>	13	41.65	1.46
Completeness [%]	98.4	100	96.2
Redundancy	9.222	9.215	8.437
CC(1/2)	0.999	0.999	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	18% PEG3350, 0.075M NaBr, 0.05M HAT buffer(combination of HEPES (7.5), ADA (6.5), and TrisHCl (8.0))