6OIC
Crystal structure of human Sulfide Quinone Oxidoreductase in complex with coenzyme Q (sulfite soaked)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-02-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.12713 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.101, 108.753, 131.058 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.846 - 2.210 |
| R-factor | 0.1839 |
| Rwork | 0.182 |
| R-free | 0.21980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.979 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.700 | 2.250 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.140 | 1.300 |
| Rpim | 0.065 | 0.588 |
| Number of reflections | 61813 | 2520 |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 95.3 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2M ammonium tartrate dibasic, 20% PEG 3350 |
