6OI4

RPN13 (19-132)-RPN2 (940-952) pY950-Ub complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-12-12
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 31
Unit cell lengths	99.373, 99.373, 41.705
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.947 - 1.760
R-factor	0.1743
Rwork	0.173
R-free	0.20710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5v1y
RMSD bond length	0.011
RMSD bond angle	1.157
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.820
High resolution limit [Å]	1.760	1.760
Rpim	0.069	0.663
Number of reflections	46327	4392
<I/σ(I)>	12.8
Completeness [%]	99.0	98
Redundancy	9.2	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.8	277	0.1 M citric acid, pH 4.6, 20% PEG6000