6OHG

Structure of Plasmodium falciparum vaccine candidate Pfs230D1M in complex with the Fab of a transmission blocking antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-11
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	79.954, 191.334, 41.198
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.049 - 2.385
R-factor	0.233
Rwork	0.229
R-free	0.27710
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.049	24.049	2.530
High resolution limit [Å]	2.385	6.890	2.390
Rmerge	0.051	0.031	0.321
Rmeas	0.057	0.035	0.363
Number of reflections	25684	1165	3756
<I/σ(I)>	17.16	33.1	3.86
Completeness [%]	98.3	96.1	91.9
Redundancy	4.663	4.184	4.41
CC(1/2)	0.999	0.999	0.957

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	15-22% PEG 4000, sodium acetate pH=4.6, 0.05-0.1 molar magnesium chloride, 20% glycerol