6OHA

Yeast Guanine Deaminase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	P 43 2 2
Unit cell lengths	106.812, 106.812, 114.178
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.070 - 2.210
R-factor	0.1584
Rwork	0.156
R-free	0.20340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ood
RMSD bond length	0.012
RMSD bond angle	1.139
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.070	2.290
High resolution limit [Å]	2.210	2.210
Rmerge	0.146	0.973
Number of reflections	33428	3075
<I/σ(I)>	10
Completeness [%]	99.1
Redundancy	8.8
CC(1/2)	0.996	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	17% Peg 3350 0.2 M ammonium sulfate 100 mM Hepes, pH 7.5