6OGN

Crystal structure of mouse protein arginine methyltransferase 7 in complex with SGC8158 chemical probe

Replaces:  6NPG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	97.813, 97.813, 169.539
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.030 - 2.400
R-factor	0.2111
Rwork	0.208
R-free	0.26122
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4c4a
RMSD bond length	0.009
RMSD bond angle	1.365
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.114	0.072	1.172
Rmeas	0.118	0.075	1.223
Rpim	0.033	0.022	0.346
Number of reflections	33363	1830	1622
<I/σ(I)>	9.5
Completeness [%]	99.7	97.4	100
Redundancy	12.9	12	12.2
CC(1/2)		0.986	0.689

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	293	20% PEG3350, 0.04 M citric acid, 0.06 M Bis-Tris propane, pH 6.4