6OFR
The crystal structure of the outer membrane transporter YddB from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 76.520, 76.520, 412.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.920 - 2.400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zgv |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.920 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.219 | 1.960 |
| Rpim | 0.072 | 0.622 |
| Number of reflections | 49176 | 4454 |
| <I/σ(I)> | 6 | 1.5 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 10.4 | 10.7 |
| CC(1/2) | 0.991 | 0.722 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 293 | 0.1 M Na cacodylate, 0.15 M Ca acetate, 15 % PEG 8000 and 20 % glycerol |
