6OEL

Engineered Fab bound to IL-4 receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.999939
Spacegroup name	F 41 3 2
Unit cell lengths	328.100, 328.100, 328.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.140 - 3.100
R-factor	0.2016
Rwork	0.199
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 3BPL & 3SOB
RMSD bond length	0.003
RMSD bond angle	0.553
Data reduction software	XDS (November 3, 2014)
Data scaling software	XDS (November 3, 2014)
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.143	82.015	3.290
High resolution limit [Å]	3.104	9.260	3.104
Number of reflections	26526	1201	4395
<I/σ(I)>	14.87	65.99	1.08
Completeness [%]	100.0	99.3	100
Redundancy	17.7	14.97	17.35
CC(1/2)	0.997	1.000	0.403

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	295	22.5% PurePEG (Microlytic), 0.1 M sodium citrate, pH 5.5, 0.6 M ammonium nitrate