6OED
CRYSTAL STRUCTURE OF THE RV144 C1-C2 SPECIFIC ANTIBODY CH55 FAB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.990, 74.030, 75.930 |
| Unit cell angles | 106.84, 108.43, 91.78 |
Refinement procedure
| Resolution | 42.116 - 2.461 |
| R-factor | 0.2589 |
| Rwork | 0.256 |
| R-free | 0.31200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qeg |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.908 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.085 | 0.697 |
| Rpim | 0.085 | 0.697 |
| Number of reflections | 73117 | 6090 |
| <I/σ(I)> | 4.9 | 1.2 |
| Completeness [%] | 76.8 | 79.4 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 294 | 25% PEG 4000 0.1 M MES pH 5.5 0.15 M ammonium sulfate |
