6OEB

Crystal structure of HMCES SRAP domain in complex with 3' overhang DNA

Replaces:  6NLD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97741
Spacegroup name	I 1 2 1
Unit cell lengths	55.728, 51.151, 149.210
Unit cell angles	90.00, 92.77, 90.00

Refinement procedure

Resolution	48.380 - 2.100
R-factor	0.2074
Rwork	0.206
R-free	0.23590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ko9
RMSD bond length	0.008
RMSD bond angle	1.527
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.380	48.380	2.160
High resolution limit [Å]	2.100	8.910	2.100
Rmerge	0.079	0.037	0.860
Rmeas	0.090	0.044	0.980
Rpim	0.042	0.022	0.462
Number of reflections	24718	354	2021
<I/σ(I)>	10.2
Completeness [%]	99.8	99.2	99.7
Redundancy	4.4	4	4.3
CC(1/2)	0.997	0.993	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% PEG3350, 0.2 M ammonium sulfate, 0.1 M HEPES