6OE4
Prefusion RSV F monomer bound by neutralizing antibody CR9501
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.628, 153.389, 155.529 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.814 - 3.300 |
R-factor | 0.2139 |
Rwork | 0.212 |
R-free | 0.25930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.870 |
Data reduction software | iMOSFLM (7.2.1) |
Data scaling software | Aimless (0.5.28) |
Phasing software | PHASER (2.6.1) |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 53.000 | 52.810 | 3.450 |
High resolution limit [Å] | 3.300 | 11.430 | 3.300 |
Rmerge | 0.394 | 0.076 | 1.757 |
Rmeas | 0.431 | 0.085 | 1.918 |
Rpim | 0.173 | 0.036 | 0.761 |
Number of reflections | 38582 | 1029 | 4628 |
<I/σ(I)> | 5.8 | ||
Completeness [%] | 99.7 | 98.1 | 99.7 |
Redundancy | 6.1 | 5.1 | 6.5 |
CC(1/2) | 0.963 | 0.995 | 0.539 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 4.2mg/mL monomeric prefusion F (DS-Cav1) + CR9501 Fab, 14.3% (w/v) PEG8000, 2.4% (v/v) 2-methyl-2,4-pentanediol (MPD), 0.1 M sodium citrate pH 5.5, 0.1 M sodium malonate pH 7.0 |