6OD1

IraD-bound to RssB D58P variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	55.938, 55.938, 300.298
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.080 - 2.000
R-factor	0.2025
Rwork	0.200
R-free	0.24500
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.938
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.100	2.100
High resolution limit [Å]	2.000	2.050
Number of reflections	3053	5272
<I/σ(I)>	25
Completeness [%]	96.9
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	magnesium chloride, PEG 8000, Tris pH 8.5