6OCX
Structure of human CIB1 in complex with peptide inhibitor UNC10245109
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.866, 33.035, 162.402 |
| Unit cell angles | 90.00, 99.85, 90.00 |
Refinement procedure
| Resolution | 27.640 - 1.900 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xo5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.920 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.640 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 63567 | 6305 |
| <I/σ(I)> | 5.57 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 294 | 0.1 M HEPES: NaOH, pH 7.5 20 % (w/v) PEG 8000 |
