6OCU
HUMAN PI3KDELTA IN COMPLEX WITH COMPOUND 29
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.998, 109.407, 142.592 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.800 - 2.770 |
| R-factor | 0.2205 |
| Rwork | 0.219 |
| R-free | 0.28730 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.106 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.800 | 3.020 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.052 | 0.599 |
| Rmeas | 0.060 | 0.684 |
| Number of reflections | 36583 | 8247 |
| <I/σ(I)> | 16.32 | 2.25 |
| Completeness [%] | 99.1 | 99.3 |
| Redundancy | 4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 16% PEG6000 0.10 M KCl 0.10 M MES pH 6.0 |
