6OCP
Crystal structure of a human GABAB receptor peptide bound to KCTD16 T1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 |
Unit cell lengths | 56.792, 69.105, 137.889 |
Unit cell angles | 102.19, 94.83, 91.15 |
Refinement procedure
Resolution | 67.110 - 2.350 |
R-factor | 0.2225 |
Rwork | 0.222 |
R-free | 0.24120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ocr |
RMSD bond length | 0.009 |
RMSD bond angle | 0.960 |
Data reduction software | XDS |
Data scaling software | Aimless (0.2.17) |
Phasing software | MOLREP |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.110 | 2.710 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.049 | 0.296 |
Rmeas | 0.070 | 0.418 |
Rpim | 0.049 | 0.296 |
Total number of observations | 149442 | |
Number of reflections | 77129 | 26932 |
<I/σ(I)> | 7.4 | |
Completeness [%] | 90.7 | 90.3 |
Redundancy | 1.9 | 1.9 |
CC(1/2) | 0.991 | 0.920 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293.15 | 2% PEG 6000 2% glycerol 8% PEG 400 0.1 M sodium cacodylate trihydrate, pH 5.6 |