6OBP

Reconstituted PP1 holoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	132.309, 69.703, 81.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.730 - 2.700
R-factor	0.2014
Rwork	0.199
R-free	0.25770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 4MOV & 6HKW
RMSD bond length	0.002
RMSD bond angle	0.431
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.730	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.112	2.035
Number of reflections	21053	2742
<I/σ(I)>	10.8
Completeness [%]	99.2
Redundancy	7.4
CC(1/2)	0.998	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	289	14% PEG4000, 6% MPD, sodium/potassium phosphate