6OBD

Crystal structure of anti-GLD52 Fab complex with human GLD52 peptide mimetic

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-04
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5419
Spacegroup name	P 21 21 21
Unit cell lengths	54.400, 131.200, 133.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.530 - 2.200
R-factor	0.2372
Rwork	0.234
R-free	0.30680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.848
Data reduction software	HKL-2000
Data scaling software	d*TREK
Phasing software	BALBES
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.530	30.530	2.280
High resolution limit [Å]	2.200	4.730	2.200
Rmerge	0.054	0.039	0.216
Rmeas	0.063	0.045	0.293
Total number of observations	149992
Number of reflections	47509	5193	4167
<I/σ(I)>	12.9	23.6	2.8
Completeness [%]	95.9	99.3	84.9
Redundancy	3.13	3.87	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.8	294	0.05M zinc acetate, 17% PEG3350, 0.1M MES pH 5.8