6OB7

Human equilibrative nucleoside transporter-1, dilazep bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2018-07-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	72.040, 72.040, 173.371
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	62.388 - 2.300
R-factor	0.2054
Rwork	0.203
R-free	0.24270
Structure solution method	SIRAS
RMSD bond length	0.006
RMSD bond angle	0.868
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.390	2.380
High resolution limit [Å]	2.300	2.300
Rpim	0.110	0.560
Number of reflections	22877	2236
<I/σ(I)>	6	1.4
Completeness [%]	91.8	82.4
Redundancy	4.6	4.1
CC(1/2)	0.990	0.390

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	9	298	50% PEG 400, 0.5 M NaCl, 0.1 M glycine pH 9.0