6OAV

JAK2 JH2 in complex with JAK146

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	44.123, 57.356, 60.744
Unit cell angles	90.00, 110.45, 90.00

Refinement procedure

Resolution	41.343 - 1.939
R-factor	0.2019
Rwork	0.200
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5usz
RMSD bond length	0.007
RMSD bond angle	0.775
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	200.000	2.060
High resolution limit [Å]	1.939	1.940
Rmeas	0.113
Number of reflections	20958	3430
<I/σ(I)>	11.7
Completeness [%]	98.6
Redundancy	5.9
CC(1/2)		0.867

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277.15	0.1M TRIS, PH 8.0 0.2M SODIUM ACETATE, 0.001 M TCEP, 12-24% PEG 4,000