6OAJ
HUaE34K 19bp SYM DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-14 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.115820 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.602, 86.353, 91.689 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.492 - 4.092 |
| R-factor | 0.3138 |
| Rwork | 0.312 |
| R-free | 0.32870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yex |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.275 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.492 | 4.238 |
| High resolution limit [Å] | 4.092 | 4.092 |
| Rmerge | 0.119 | 1.942 |
| Rmeas | 0.132 | 2.129 |
| Rpim | 0.055 | |
| Number of reflections | 4331 | 406 |
| <I/σ(I)> | 7.28 | 1.01 |
| Completeness [%] | 99.3 | 98.54 |
| Redundancy | 6.2 | 6 |
| CC(1/2) | 0.996 | 0.479 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1M Na-malonate, pH 5.0, 12% PEG 3350 |
