6OA8

Superfolder Green Fluorescent Protein with 4-cyano-L-phenylalanine at the chromophore (position 66)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 41 2 2
Unit cell lengths	55.266, 55.266, 166.483
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.450 - 1.370
R-factor	0.1627
Rwork	0.161
R-free	0.18850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.010
RMSD bond angle	1.130
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	165.760	1.390
High resolution limit [Å]	1.370	1.370
Number of reflections	55127	2740
<I/σ(I)>	20.7	1.2
Completeness [%]	99.6	96.1
Redundancy	13.4	7.6
CC(1/2)	0.999	0.393

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	295	0.1 M Tris-HCl, 2.0 M ammonium sulfate, pH 8.5