6O8Q

HUaa 19bp SYM DNA pH 4.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.115830
Spacegroup name	P 21 21 21
Unit cell lengths	59.411, 61.151, 351.203
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.180 - 3.216
R-factor	0.2504
Rwork	0.247
R-free	0.28540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.692
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.180	3.331
High resolution limit [Å]	3.216	3.216
Rmerge	0.177	1.388
Rmeas	0.185	1.444
Rpim	0.053
Number of reflections	20484	1608
<I/σ(I)>	11.31
Completeness [%]	93.9	73.05
Redundancy	12.3
CC(1/2)	0.999	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293.15	0.1 M Na-malonate, pH 4.0 12% PEG 3350