6O8E

Crystal structure of UvrB bound to duplex DNA with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979200
Spacegroup name	P 2 21 21
Unit cell lengths	69.269, 115.719, 266.795
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.510 - 2.610
R-factor	0.2175
Rwork	0.215
R-free	0.26980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.394
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.510	2.670
High resolution limit [Å]	2.610	2.610
Number of reflections	65131	4600
<I/σ(I)>	11.8
Completeness [%]	99.5
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	100 mM Bis-Tris, pH 6.5, 100 mM ammonium acetate, 17-19% w/v PEG8000