6O7B

Crystal structure of Csm1-Csm4 cassette in complex with cA4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 65 2 2
Unit cell lengths	156.578, 156.578, 187.087
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.480 - 2.400
R-factor	0.22646
Rwork	0.222
R-free	0.27118
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mua
RMSD bond length	0.005
RMSD bond angle	1.527
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rpim	0.025
Number of reflections	52979	5181
<I/σ(I)>	27.7
Completeness [%]	99.9
Redundancy	37.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	293	0.1 M phosphate-citrate pH 4.2, 5% PEG3000, 25% 1,2-propanediol, and 10% glycerol