6O6T

Crystal structure of Csm6 H132A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	C 2 2 21
Unit cell lengths	104.371, 163.271, 111.016
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.300 - 2.310
R-factor	0.24122
Rwork	0.239
R-free	0.28471
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6o6s
RMSD bond length	0.002
RMSD bond angle	1.216
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rpim	0.057
Number of reflections	41027	1896
<I/σ(I)>	10.2
Completeness [%]	99.8
Redundancy	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	10% PEG 8K, 0.2 M NaCl, 0.1 M Na/K phosphate pH 6.2