6O6M
The Structure of EgtB (Cabther)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97648 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.294, 137.315, 85.391 |
| Unit cell angles | 90.00, 92.43, 90.00 |
Refinement procedure
| Resolution | 45.855 - 2.506 |
| R-factor | 0.1865 |
| Rwork | 0.185 |
| R-free | 0.22570 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.656 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.860 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 67215 | 4473 |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.1M Na acetate 0.1M Tris acetate 5% PEG 4000 |
