6O5L

Crystal structure of PprA filament from Deinococcus peraridilitoris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 64 2 2
Unit cell lengths	79.468, 79.468, 262.804
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	66.576 - 3.300
R-factor	0.2489
Rwork	0.247
R-free	0.29860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mc8
RMSD bond length	0.008
RMSD bond angle	1.059
Data reduction software	iMOSFLM (7.2.2)
Data scaling software	Aimless (0.7.1)
Phasing software	PHASER ((1.13_2998: ???))
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.820	3.560
High resolution limit [Å]	3.300	3.300
Rpim	0.075
Number of reflections	8106	1591
<I/σ(I)>	10.7	2.3
Completeness [%]	100.0	100
Redundancy	32.9	35.4
CC(1/2)	0.999	0.507

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293.15	54 uM protein, 5% w/v PEG8000, 500 mM sodium chloride, 75 mM imidazole, 10 mM Tris, 50 mM potassium phosphate, 10 mM trimethylamine-HCl