6O5H
The effect of modifier structure on the activation of leukotriene A4 hydrolase aminopeptidase activity.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-05-23 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 |
| Unit cell lengths | 139.663, 139.663, 84.149 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 120.950 - 2.840 |
| R-factor | 0.2191 |
| Rwork | 0.219 |
| R-free | 0.22860 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.951 |
| Data reduction software | PROTEUM |
| Data scaling software | PROTEUM |
| Phasing software | PROTEUM |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 120.950 | 2.914 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Number of reflections | 40540 | |
| <I/σ(I)> | 4.02 | |
| Completeness [%] | 98.6 | |
| Redundancy | 6.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 50 - 80 mM magnesium formate dihydrate and 24% - 29% PEG3350 |
