6O41

Crystal structure of the unbound PGZL1 germline Fab fragment (PGZL1_gVmDmJ)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	78.840, 107.490, 131.960
Unit cell angles	90.00, 94.61, 90.00

Refinement procedure

Resolution	39.670 - 2.465
R-factor	0.1714
Rwork	0.169
R-free	0.21870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6o3d
RMSD bond length	0.005
RMSD bond angle	0.820
Data reduction software	XDS (20180126)
Data scaling software	XSCALE (20180126)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.670	2.530
High resolution limit [Å]	2.465	2.465
Rpim	0.062	0.597
Number of reflections	77485	5460
<I/σ(I)>	9.2
Completeness [%]	97.7	94
Redundancy	3.5	3.4
CC(1/2)	0.995	0.526

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	20% PEG3350, 0.2 M lithium citrate